Product Name:
Rabbit polyclone antibody against grouper(E. coioides) IgM H chain Puriified Ig, lyophiliczed protein
Catalog Number: GDN‐A0004
Size: 50ug, lyophilised protein
Reconstitution: Add 200 μl of distilled water
Lot Number: See label on vial
Host: Rabbit polyclonal
Species reactivity: Grouper (E.Coioides)
Cross‐reactive species:
- Epinephelus coioides × Epinephelus malabaricus (青斑)
- Epinephelus coioides × Epinephelus lanceolatus (青龍班)
- Epinephelus lanceolatus (龍膽石斑)
- Epinephelus lanceolatus × Epinephelus fuscoguttatus (龍虎斑)
- Epinephelus tukula (藍身大石斑)
- Cromileptes altivelis (駝背鱸、老鼠斑)
Application:
- Western Blot : 20000X
- ELISA: 20000X
- Immunocytochemistry (ICC): 10000X
ELISA:
- Coating: Coat 5μg of purified gIgM protein in the ELISA plate at 37℃ for 2 hours.
- Blocking: Block with gelatin‐NET solution at room temperature for 1~2 hours. Wash with PBST for three times.
- First antibody: Incubate with rabbit anti‐gIgM serum in adequate concentration (dilute with gelatin‐NET solution) at 37℃ for 30 min and 4℃ for 1 hour. Wash with PBST for three times.
(Pre‐immune serum: 2000X, immune serum: 2000, 4000, 8000X) - Secondary antibody: Incubate with goat anti‐rabbit Ig conjugated with HRP inadequate concentration (2000X) at 37℃ for 30 min and 4℃ for 1 hour. Wash with PBST for five times.
- Substrate solution: Incubate with OPD substrate solution at 37℃ for 30 min. Measure the absorbance at 405 nm wavelengths and 650 nm wavelengths as background by ELISA reader.
Ig against grouper IgM H chain (gIgM H) was purified form the rabbit antiserum and lyophilized. Therefore, we recommend to dissolve the lyophilized Ig in 200μL ddH2O and dilute it for about 20,000~40,000 fold.
Description:
The optimal dilution factor of rabbit antiserum against grouper IgM H‐chain in western blot was no less than 8000‐40000X. In a separate assay, the optimal dilution factor of antiserum for ELISA was 20000X (data not shown).
Application Notes:
The following concentration ranges are recommended starting points for this product. The investigator should determine the optimal working concentrations for specific applications.
Storage:
For longer periods of storage, store at ‐20°C. Avoid repeat freeze‐thaw cycles.
This product is for in vitro research use only.
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